Like it or not NE has a culture and so does Pittsburgh. I guess I am saying yes.
Except for the many documented times in which the Steelers were Team Dissension. Just a few quick examples from the Mike Tomlin era:
I know, I know ... the response will be "but this proves the Steelers have a strong culture. They overcame the typical locker room problems that every NFL team has and continued to put a winning product on the field." I consider this to be an example of what is called the "narrative fallacy" - "our tendency to construct stories around facts, which in love for example may serve a purpose, but when someone begins to believe the stories and accommodate facts into the stories, they are likely to err." The Steelers continue to win; there must be something more to it than having a future HOF QB, stable ownership, and stable/sound coaching. We'll call that "culture." At some point things will go south (they always do) and Mike Tomlin will quit or retire or be kicked upstairs (perhaps coinciding with their QB retiring?) and all of a sudden there won't be a winning culture there after all. It's all circular.
Now, I'm willing to concede this: there may be organizations (businesses, sports teams, even governments - some would say Singapore?) that are so strong that they instill in their young employees the same ethic of hard work, stability, and sound fact-based decisionmaking that they are able to sustain excellence even when key personnel retire or otherwise leave. I will grant that. But you'd have to show me a sports team that manages to sustain excellence in this situation. The Steelers are about as good an example as I can think of (mostly due to a remarkable 3 head coaches in 45+ years, but even there Cowher came from outside the Steelers (the Browns) and Tomlin too (Bucs/Vikes), so it isn't a good example of coming up through the system learning the "Steelers way." On the other hand, the examples of an Assistant taking over when a head coach retires aren't very compelling; Richie Pettibone following Joe Gibbs' first retirement comes to mind as a colossal failure of promoting from within.
So after all that ... no, I'm not convinced that there is something real that we can call "a winning culture" ...